European Journal of Biomedical and Pharmaceutical Sciences

EMBRYOGENIC CALLUS INDUCTION AND PLANTLET PROLIFERATION OF SOLANUM NIGRUM (L.) THROUGH LEAF EXPLANTS

Venkateshwarlu Mandaloju*

ABSTRACT

In the present investigation, in vitro plant let proliferation from embryogenic callus of two-month-old Solanum nigrum L. was achieved employing leaf explants. Establishment of efficient protocol for the induction of somatic embryogenesis and plantlet regeneration from leaf explants was achieved in Solanum nigrum L. Highest frequency (89%) of embryogenic callus formation was obtained from leaf explants of Solanum nigrum L. on Murashige and Skoog (MS) medium fortified with different concentration of 2,4-Dichlorophenoxy acetic acid. Fast growing, greenish nodular callus lines containing somatic embryos were established on initiation medium supplemented with (2.5mg/L) 2.4-D respectively. Embryo development and maturation was achieved on MS medium supplemented with (2.5mg/L) 2,4-D and (2.0 mg/L) TDZ. The well formed embryos germinated into complete plantlet on MS medium containing (1.0mg/L) 2.4-D in combination with (2.0mg/L) TDZ. The regenerated plants ware first transferred to plastic cups and later into pots for maturation.

Keywords: Leaf explants, embryogenic callus, somatic embryogenesis, plantlet proliferation.