Abstract
DIVERSITY AND ENZYMATIC ACTIVITY OF ENDOPHYTIC FUNGI ISOLATED FROM PINE APPLE (ANANASA COMOSUS)

Prithviraj Sadar*, Navnath Jadhav, Nikhilesh Kulkarni and Rachana Pachori

ABSTRACT

Endophytes are the microorganisms includes Bacteria, Fungi and Actinomycetes that are present in living tissues of various plants without causing any symptom of diseases. Endophytes are untapped source of bioactive compounds can offer enzymes production for Pharmaceutical, Leather, Detergents, Agriculture and textile industries. Hence, the present work planned to evaluate the production of extracellular enzymes viz. Amylase, Protease and Lipase from fungal endophytes of Crown leave and Fruit pulp of pine apple. Total 28 fungal endophytes of the genera Aspergillus, Penicillium, Cladosporium, Fusarium, Colletotrichum, and Alternaria were isolated and assessed for extracellular enzyme production. The enzyme activity was analyzed by hydrolysis of substrate on solid medium adopting spot inoculation method. The results showed that all the fungal isolates were able to produce one and other tested enzymes.

Keywords: Endophytes, Extracellular Enzyme, Substrate hydrolysis, Bromelain, Ananasa comosus.


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