PSEUDOMONAS AERUGINOSA: ISOLATION, CHARACTERIZATION AND EVALUATION OF LARVICIDAL EFFICACY AGAINST AEDES AEGYPTI L.
Rajiv Kumar Shukla, Dr. Sarita Kumar* and Pushplata Tripathi
ABSTRACT
The present study investigates the bioactivity of Pseudomonas aeruginosa against the fourth instar larvae of Aedes aegypti. The bacterium was isolated from the soil samples collected from various parts of Delhi and characterized based on their biochemical and morphological characters. The identification of bacterium was carried out with the help of MALDI-TOF MS Biotyper which confirmed the bacterium as Pseudomonas aeruginosa. The mosquitocidal potential of the isolated bacteria was evaluated against early fourth instars of dengue vector, Aedes aegypti maintained in the laboratory under controlled conditions of 28 ± 1 °C and 80 ± 5 % RH with a photoperiod of 14h daylight/10h darkness. The bioassay was performed in accordance with the protocol described by World Health Organization which proved the significant larvicidal efficacy of Pseudomonas aeruginosa against early fourth instars of Ae. aegypti. The larval exposure to bacterial formulation for 24 h resulted in the respective LC50 and LC90 values of 5.58 and 12.80 mg/mL. The present study concludes that non-pathogenic bacteria present in the natural environment can be used as potential bio-control agents against the larvae of Aedes aegypti. Further studies are needed to identify the bioactive extracellular and intracellular metabolites of bacteria effective against dengue vector larvae.
Keywords: Pseudomonas aeruginosa, Aedes aegypti, larvicidal, MALDI-TOF.
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