EVALUATION OF ANTIHISTAMINIC ACTIVITY OF OCIMUM SANCTUM SEEDS AQUEOUS EXTRACT IN GUINEA PIG.
Bari Padma*, Kanakam Vijayabhaskar, Mathukumalli Sai Laxmi and Kalakota Chaitanyaprasad
ABSTRACT
In the present study, the pharmacological evaluation of aqueous extract and essential oil extract of seeds has been done for their antihistaminic activity on guinea pig. In isolated guinea pig tracheal chain preparation, there was a right side shift of dose response curve (DRC) of histamine. Chlorpheniramine maleate was used as a standard drug. showed the result of aqueous extract and essential oil of Ocimum sanctum (100,200 mg/kg) significantly protected the Guinea pigs against histamine-induced bronchospasm. The guinea pigs exposed to histamine aerosol showed signs of progressive dyspnoea leading to convulsions. The aqueous extract and essential oil of Ocimum sanctum significantly prolonged the latent period of convulsions (PCT as compared to control following the exposure of histamine aerosol. The action started after 1 h of drug administration. The antihistaminic drug Chlorpheniramine maleate used in the study produced a significant increase in the latent period of convulsion after 1 h. Essential oil (100 ml/kg)and 200mg/kg % protection i.e. 80.48 and 95.5 their onset of convulsion in secondary 606.2. ±0.359**, 895.1 ±0.201** and aqueous extract 100mg/kg, 200mg/kg 304.0 ±.0.391**, 352.6 ±0.401** %protection 78.0, 88.48. Moreover extracts of Ocimum sanctum disturbed histamine aerosol induce bronchoconstriction in whole guinea pig, where essential oil was more effective comparatively to aqueous extract. Thus from the results obtained in the present investigation, it can be concluded that aqueous extract and essential oil of Ocimum sanctum possess antihistaminic activity.
Keywords: Ocimum sanctum seeds, H1-antagonist, guinea pig.
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