European Journal of Biomedical and Pharmaceutical Sciences

ESTIMATION OF TOTAL PHENOLICS, FLAVONOID AND IN VITRO ANTIOXIDANT ACTIVITY OF CHROZOPHORA ROTTLERI

Kavitha G. C., Riaz Mahmood* and Pallavi M.

ABSTRACT

The present study was contemplated to evaluate the total polyphenols, flavonoids and in vitro antioxidant potential of Chrozophora rottleri Leaf extracts. The dried leaves were subjected to soxhlet extraction utilizing chloroform and methanol followed by preliminary qualitative phytochemical analysis. Total phenolic content was estimated by Folin-Ciocalteu method and Aluminium chloride method was used for flavonoid estimation. The in vitro antioxidant assays were determined by using various radical scavenging activities such as DPPH, superoxide anion, nitric oxide, hydroxyl, ferrous ion chelating activity and reducing power assay at different concentrations. The results of the total phenolic and flavonoid content estimation was expressed in terms of equivalence with standard and the antioxidant potentiality of various scavenging and chelating activity were expressed in terms of IC50values. The results showed that both methanol and chloroform extracts possess significant antioxidant activity. However methanol extract, was found to be superior to the chloroform extract in terms of parameters assessed. The study therefore suggests Chrozophora rottleri leaves, as a significant source of natural antioxidants, which might be helpful in preventing or slowing the progress of various oxidative stress- related diseases.

Keywords: Chrozophora rottleri, in vitro antioxidant activities, radical scavenging, total phenolics and flavonoids.