European Journal of Biomedical and Pharmaceutical Sciences

COMPARISON STUDY BETWEEN THREE NON INVASIVE METHODS USED FOR DIAGNOSING HELICOBACTER PYLORI INFECTION

Ahmed Elsheikh Saleem Mohamed Khair, Mohammed Gaffar Mohammed Ibrahim, Mohamed Assadig Ahmed Hussein* and Elamin Mohamed Ibrahim

ABSTRACT

Background: Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium it is one of the most common human-specific pathogens which exclusively inhabits the gastric mucosa. Infection with H. pylori is always associated with chronic gastric inflammation, gastritis and peptic ulceration which can lead to gastric cancers such as adenocarcinoma. The aim of this study was to compare between three non invasive methods used for diagnosing Helicobacter pylori infection. Methodology: this study was conducted in Khartoum state from February to May 2017. Stool and serum samples were collected from 105 patients with gastric disorders. The presence of H.pylori infection was evaluated by urea breath test, stool antigen detection test and antibody detection test. Results: A total number of 45 patients gave positive result in urea breath test while 60 patients' showed negative result.44 patients gave positive result in stool antigen test while 61 patients showed negative result. A total number of 40 patients gave positive result in antibody detection test while 65 patients' showed negative result. The result showed that the similarity between urea breath test, stool antigen test, urea breath test, antibody detection test and stool antigen test, antibody detection test was 99%, 72,4% and 71,4 % respectively. While the similarity between these three method was 69.5%. Conclusion: In this study there was no significant differences between these three non invasive methods. The combination of antibody detection and stool antigen test or using urea breath test only can significantly improve the detection of H. pylori infection.

Keywords: H. pylori, urea breath test, stool antigen test, antibody detection test.