European Journal of Biomedical and Pharmaceutical Sciences

STABILITY INDICATING CHEMOMETRIC METHODS FOR DETERMINATION OF CEFACLOR IN PRESENCE OF ITS ACID INDUCED DEGRADATION PRODUCT.

Khalid A.Mahmoud. Attia, Nasr M. El-absawy, Ebrahim Abolmagd*

ABSTRACT

Three simple, accurate and Precise multivariate calibration methods, including classical least square (CLS), principal component regression (PCR) and partial least square (PLS), have been used for the determination of Cefaclor (CFC) in the presence of its acid induced degradation product. The CLS, PCR and PLS techniques are useful in spectral analysis because the simultaneous inclusion of many spectral wavelengths instead of the single wavelength used in derivative
spectrophotometry has greatly improved the precision and predictive abilities of these multivariate calibrations. (CFC) was analyzed with mean accuracies 100.08+1.83, 100.09+1.81 and 100.1+1.82 for CLS, PCR and PLS, respectively. The developed methods were statistically compared with a reported method and no significant differences were observed regarding both accuracy and precision, all the developed methods have been validated according to ICH guidelines.

Keywords: Stability-indicating; Cefaclor; PLS; PCR; CLS.