European Journal of Biomedical and Pharmaceutical Sciences

IN VIVO HEPATOPROTECTIVE EFFECT OF ETHANOLIC EXTRACT OF CAPSICUM ANNUUM L. RED VARIETY AGAINST PARACETAMOL INDUCED HEPATOTOXICITY IN MALE WISTAR ALBINO RATS

A. Sathya Priya* and A. Anitha

ABSTRACT

This study was designed to evaluate the hepatoprotective activity of ethanolic extract of Capsicum annuum L. Red variety (C. annuum) against paracetamol induced liver damage in male wistar albino rats. The ethanolioc extract was administered orally to the wistar albino rats with hepatotoxicity induced by paracetamol. Silymarin was given as reference standard. The plant extract was effective in protecting the liver against the injury induced by paracetamol in rats. The liver injury was induced by paracetamol@750mg/kg b.w orally. The rats were divided in five groups. Rat of groupG1 served healthy control receive no treatment. G2 served as toxic control and receive paracetamol orally @750mg/kg at every 72h interval for 21d. G3 received with standard silymarin 50mg/kg for 21d and simultaneously administered paracetamol. G4 received with ethanolic extract of C. annuum 250mg/kg for 21d and simultaneously administered paracetamol. G5 received ethanolic extract of C. annuum 500mg/kg for 21d and simultaneously administered paracetamol. Various biochemical parameters like lipid content, free fatty acids, triglycerides, phospholipids, glycoprotein, hexose, hexosamine, sialicacid and glycogen were estimated liver tissue of treated and untreated animals. In serum, total protein, albumin, aspartate transaminase, alanine transaminase, alkaline phosphatase and total bilirubin were analyzed using commercially available test diagnostic kits. This was evident from significant reduction in serum enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP). Our results confirmed that the ethanolic extract of C. annuum possesses hepatoprotective activity against paracetamol induce hepatotoxicity rats.

Keywords: Hepatoprotection, Paracetamol, Capsicum annuum L, Silymarin.