European Journal of Biomedical and Pharmaceutical Sciences

EVALUATION OF CYTOTOXIC ACTIVITY OF ZANTHOXYLUM ACANTHOPODIUM IN DALTON’S LYMPHOMA ASCITES CELLS INDUCED CANCER IN MICE

Joyeeta Bhattacharya*, Aparna Lakshmi I., Sudipta Ata, Oly Sen Sarma

ABSTRACT

Objective: The quest for plants with valuable medicinal properties continues to receive keen attention by many scientists for biological activities, which range from antibiotics to antitumor agents. The present study demonstrates dose dependent anti-cancer effect of Zanthoxylum acanthopodium L. fruit extracts by in-vivo methods scientifically with an objective to overcome cancer. Methods: The anti-cancer activity of the aqueous extract of Zanthoxylum acanthopodium (AEZA) and methanolic extract of Zanthoxylum acanthopodium (MEZA) were tested against Dalton’s Lymphoma Ascites (DLA) cell lines induced cancer in Swiss albino mice. The degree of protection was determined by change in body weight (gm), Percentage decrease in body weight (%), Tumour volume (ml), Packed cell volume (ml), Percentage cell viability (%), Haematological parameters (R.B.C, W.B.C and Hemoglobin content), Mean survival time (MST), % increase in lifespan (% ILS) and histopathological parameters. Results: AEZA and MEZA (200 mg/kg and 400mg/kg per oral (p.o)) have shown significant prolongation of lifespan, reduction in tumor volume, packed cell volume, cell viability, and improvement in the hematological parameters, changes in histopathological observation when compared to the rest of the groups. AEZA and MEZA at 400mg/kg b.w p.o. possess better anticancer activity than rest of its doses of other drug. The results of the present study are encouraging, as both the extracts have shown significant anticancer activity. Conclusion: The results indicate that the AEZA and MEZA (200 mg/kg and 400mg/kg b.w, p.o possess significant antitumor activity and its potency to be developed as an anticancer agent.

Keywords: Anti-Cancer; Zanthoxylum acanthopodium, Dalton’s Lymphoma Ascites (DLA), Mean survival time (MST).