European Journal of Biomedical and Pharmaceutical Sciences

EVALUATION OF SPERMICIDAL ACTIVITY ON ROOT METHANOLIC EXTRACT N-BUTANOL FRACTION OF ANDROGRAPHIS PANICULATA (FAMILY- ACANTHACEAE)

Kanakam Vijayabhaskar*, Syed Saleem ahmed, Dr. M.Aruna Devi  and Bhukya Nageshwarrao

ABSTRACT

Andrographis paniculata (Burm.f.) Wall. ex Nees., (Family- Acanthaceae) (English name-King of Bitters, Tamil name-Nilavempu) is an annual herbaceous plant and is extensively cultivated in Southern Asia, China and some parts of Europe. The aim of this study is to highlight the work on plant drugs and their bioactive extracts involved in male anti-fertility mechanism. The aqueous extract of Andrographis paniculata. In the present study we have evaluated the effective spermicidal concentration of this extract on male albino rat sperm, by conducting “Sander-Cramer test”. The minimum effective spermicidal concentrations of Andrographis paniculata root extract butanol fraction were found to be 12±0.26 mg/ million sperm. After there were no morphological changes observed in the head, mid –piece & tail of sperm. In the in-vivo study, a dose dependent reduction in the epididymal sperm count and percentage motility were observed. These results showed that Andrographis paniculata butanol fraction has antifertility effect on male rat reproduction, sexual behavior and epdidymal sperm concentration. So butanol fraction of Andrographis paniculata root is a potent spermicide which completely immobilized the one million of rat sperm within 20s.

Keywords: Andrographis paniculata, Spermicidal, Sperm motility, Sperm viability.