European Journal of Biomedical and Pharmaceutical Sciences

GENETIC FUSION AND CONFORMATION OF CHOLERA TOXIN B GENE WITH P CAMBIA 1301 IN AGROBACTERIUM TUMEFACIENS (LBA4404)

R. Saravanan* D.Dhachinamoorthi, Y. Sudhakar

ABSTRACT

Expression of antigens to be used as vaccines, against of pathogens in transgenic plants is a convenient and inexpensive source for these immunotherapeutic molecules. In addition, developing safe vaccines has been another important objective in vaccine research since conventional vaccines having viable attenuated microbial components pose small risks to the world population. For this reason, vaccine makers today favour subunit vaccines that contain primarily the antigenic epitopes from pathogens as vaccine component instead the whole attenuated live or killed pathogens in the vaccines. These antigenic epitopes have no way of establishing an infection but offer production against infectious pathogens. In this study, CT-B gene was cloned into plant expression vector. The plasmid pCAMBIA was constructed by inserting the coding region for CT-B from PRK2013 together with pBluescript II KS between the Bam H1 and EcoRI in the sites of PGA643 plant transformation vector and expressed in the plasmid (pCAMBIA). This system was used to transform recombinant gene in to plant cells by using Agrobacterium tumefaciens, and regenerated plants, their parts used as edible vaccine.

Keywords: Cholera, CT-B, p CAMBIA, Genetic fusion, p Bluescript SK+, Vibrio cholera.