European Journal of Biomedical and Pharmaceutical Sciences

PHYTOSYNTHESIS AND IN VITRO EVALUATION OF BIOLOGICAL ACTIVITIES OF SILVER NANOPARTICLES USING CARALLUMA SARKARIAE ROOT EXTRACT

Murugapandi Murugan and Muniyappan N.*

ABSTRACT

Nanomedicine is the medical application of nanotechnology, one of the most interesting areas of research in modern material science. In the present study an eco friendly, green, low-cost, synthesis of silver nanoparticles is reported by one-pot microwave synthesis using Caralluma Sarkariae root aqueous extract used both as a reducing as well as a stabilizing agent and also evaluated their in vitro antioxidant, anti-bacterial and anti-inflammatory activities. The properties of the CSR-AgNPs were characterized by various techniques such as UV/Vis, FT-IR, SEM and HR-TEM which exhibited Surface Plasmon Resonance at 430 nm. The synthesized AgNPs are observed to be spherical in shape and in the size of 202 nm, the stability upto 6 months. Pharmacological activities of CSR-AgNPs were evaluated and showed a high antioxidizing activity by DPPH method, anti-bacterial activity by Disc diffusion method, and anti-inflammatory activity by HRBC method compared to that of standard and CSR aqueous extract.

Keywords: Caralluma sarkariae root, Microwave oven, Silver nanoparticles, HR-TEM, antioxidant, anti-inflammatory, antibacterial activity.