ENDOTHELIAL NITRIC OXIDE SYNTHASE GENE T-786C POLYMORPHISM AMONG MYOCARDIAL INFARCTION (MI) PATIENTS IN KHARTOUM, 2016
Magdolin Mustafa AboZeid and Ibrahim Khidir Ibrahim*
ABSTRACT
Background: A single nucleotide polymorphism (T > C) rs2070744 due to transition of a thymine to a cytosine at T-786C in the promoter region of eNOS was found to reduce the rate of mRNA transcription by 50%, resulting in decreased serum NO levels which can inhibit apoptosis or stimulate tumor proliferation, angiogenesis and metastasis. Objective: To evaluate the association between Nitric Oxide Synthase gene T-786C polymorphism and Myocardial Infarction patients in Sudan. Material and Methods: A total of 40 patients with myocardial infarction and 50 control subjects were enrolled this study. Blood samples were collected from all patients in EDTA. Genomic DNA was extracted from all blood samples using Guanidine chloride methods. Analysis of the eNOS786T > C promoter polymorphism were carried out by allele specific polymerase chain reaction method (PCR). Results: The present study reported that, molecular analysis showed that, the most frequent genotype in patients was TT 87.5% (35) followed by TC genotype 12.5% (5).While in the control group there was 100% TT genotype. Conclusion: The present study indicates the lack of association between eNOS and myocardial infarction.
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