European Journal of Biomedical and Pharmaceutical Sciences

EFFECTS OF BENFOT?AM?NE AND COENZYME Q10 ON GENTAM?C?N-?NDUCE TEST?CULAR TOX?C?TY AND OX?DAT?VE STRESS ?N RATS

Selda Telo*, Mehmet Alperen Üstüner, Neriman Çolako?lu, Dilara Kaman, Funda Gülcü Bulmu?

ABSTRACT

In this study, the protective effects of Benfotiamine (BFT) and Coenzyme Q10 (CoQ10) against Gentamicin (GM)-induced testicular toxicity in rats were investigated by employing the assessment of Hydroxynonenal (HNE) and asymmetric dimethylarginine (ADMA), including histopathological examination of testis tissues. Rats were divided into 4 groups randomly and treated as follows: (1) controls, (2) GM group; intraperitoneally (i.p.) injected with GM for 8 days (80 mg/kg/day), (3) GM+ CoQ10 group; treated with GM and CoQ10 (10 mg/kg/day, i.p.) for 8 days and (4) GM+BFT group; treated with GM and BFT (oral gavage 100 mg/kg/day) for 8 days. All of rats were euthanized by cervical decapitation on the eighth day of experiment. Blood samples were extracted to be used in biochemical analyses and testes were removed to be used in histopathological examinations. The serum levels of ADMA were examined by using high-performance liquid chromatography and serum HNE levels were investigated by ELISA technique. In the serum ADMA and HNE levels significant elevation and important histopathological alterations were observed in GM injected group. Serum ADMA and HNE levels were significantly decreased in BFT+GM and CoQ10+GM groups in comparison with the GM group. CoQ10 and particularly BFT treatment significantly decreased the histopathological injuries of testicular tissue due to GM toxicity. The acquired results in this study indicated that CoQ10 and particularly BFT supplement decreased gentamicin-induced testes injury via the betterment of oxidative stress of testes tissue.

Keywords: Gentamicin; testicular toxicity; coenzyme-Q10; benfotiamine; asymmetric dimethylarginine; 4-hydroxynonenal.