HISTOMORPHOLOGICAL RESPONSE OF SULFORAPHANE AND RIBOCEINE ON ANNULAR PUNCTURE- INDUCED MODEL OF RABBITS INTERVERTEBRAL DISC DEGENERATION
Ogunlade B., Osinubi A. A., Duru F. I. O., Adelakun S. A.,* Ogunlade S. A., Alao A. A.
ABSTRACT
Background: Intervertebral disc degeneration is one of the leading causes of Low back pain that is regarded as one of the most frequent neurological ailments. Objective: To determine the regenerative potential of sulforaphane and riboceine supplements in a rabbit model in annular punctured intervertebral disc degeneration. Methods: Forty New Zealand white rabbits (3 to 3.5 kg each) underwent annular puncture of the L2-L3, L3-L4, and L4-L5 discs. Rabbits were sacrificed at 2, 4 and 8 after puncture. For a longitudinal study to assess changes in disc height over time, serial X-rays were performed at 0, 4 and 8 weeks for the rabbits. Upon sacrifice, the whole spinal column and discs were extracted and analyzed with real time reverse transcriptase-polymerase chain reaction, and histological staining. Results: The radiology assessment revealeda significant progressive decrease in disc height which was reversed by the administration of sulforaphane and riboceine. Significant disc space narrowing compared to preoperative disc height was observed during the time period (p<0.05). The histological grade, collagen type 1 and 2, aggrecan, and matrix metalloprotease-13 mRNA expression and Hematoxylin and Eosin were indicative of degeneration, supporting the results of the radiology. Conclusion: Administration of sulforaphane and riboceine in this study revealed the regenerative potential of nutritional supplement in intervertebral disc. The annular puncture model can be used to study and validate the therapeutic potential of drugs and biological materials in the treatments of degenerative disc disease.
Keywords: Animal model, intervertebral disc degeneration, sulforaphane, riboceine, histology, radiology.
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