European Journal of Biomedical and Pharmaceutical Sciences

INDUCTION OF DIABETES BY ALLOXAN IN MALE WISTAR ALBINO RATS –A SIMPLIFIED METHODOLOGY

Pasupuleti Sreenivasa Rao* and Gangapatnam Subramanayam

ABSTRACT

Diabetes mellitus (DM) is burning problem, currently administered therapeutic options are major failure and new therapeutics are required. Globally herbal medicines have been recommended for its treatment, however, proper validation is required before its administration. The best option is to validate in experimental animals.Hence the current investigation is carried in aim to identify the suitable concentration of the alloxan for inducing DM in animal models, in order to avoid unnecessary pancreatic tissue damage and also to develop an experimental animal model to investigate the induction of diabetes mellitus (DM).Thirty eight, Healthy male wistar albino rats were selected andassigned to four groups: Group-II, III and IV, received intraperitoneally with varying concentrations of Alloxan (31.25, 62.5, 87.5 100, 125mg/kg), dissolved in various buffers, that include Citrate buffer, PBS buffer, and NaCl buffer respectively, whereas the experimental rats in Group-I(control) received with water and buffers only. Using a glucometer, fasting blood glucose (FBG) levels were assessed on days 0, 3, 4, and 5 respectively.Out of the 30 rats administered with alloxan, the combination of Alloxan + NaCl buffer worked efficiently, and results exhibit elevated levels of blood glucose which clearly confirms induction of DM.

Keywords: Animal Model, Alloxan, Diabetes Induction.