DETECTION OF GLUTATHINE S-TRANSFRASE M1 AND GLUTATHINE S-TRANSFRASE T1 NULL POLYMORPHISM AMONG SUDANESE PATIENT WITH SICKLE CELL ANAEMIA
Abeer Abden Ahmed Mohamed and *Ibrahim K. Ibrahim
ABSTRACT
Background: Glutathione S-transferase (GST) enzymes that play a key role in detoxification of activated carcinogens are shown to be one of the potential modifiers of individualized risk for several cancer types. Objective: This purpose of this study was to investigate the frequency of the GSTM1 and GSTT1 null polymorphism in sickle cell anemia in Sudan. Materials and methods: This study is a case control study, conducted in Khartoum state during the period from May- to July 2017. A total of 40- patients with SCA were enrolled in this study. Two and half milliliter (ml) of EDTA anticoagulated blood was collected from each patient; DNA was extracted by salting out method. The GSTM1 and GSTT 1 genotype were determined by multiplex polymerase chain reaction (PCR). Results: The rate of GSTM1 null mutation was 77% in children with SCD, while it was 40% in the control group. The frequency of individuals carrying the GSTT1 null mutation was higher in SCD patients (38%) compared to controls (22%). Conclusion: The study demonstrated that Egyptian SCD patients have high frequency of GSTT1 and GSTM1 gene polymorphism.
Keywords: Sickle Cell Anemia; Glutathione S-transferase Mu 1; null polymorphism.
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