European Journal of Biomedical and Pharmaceutical Sciences

INVESTIGATION OF THE ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF PHYLLANTHUS ACIDUS IN COMBINATION WITH DIFFERENT CLASSES OF ANTIBIOTICS AGAINST SINGLE AND MULTIDRUG RESISTANCE STRAINS

Md. Shariful Islam*, Tawfiqa Rahman, Md. Hasan-Al Jubayed, Pankoj Chondro Roy, Md Abdur Rahaman Miah, Abdullah-Al-Mamun, Md. Ibrahim Sunny

ABSTRACT

The aim of this project work was to evaluate the Antimicrobial Activities of phyllanthus acidus (Family: Phyllanthaceae). Antimicrobial activity of chloroform and ethyl acetate extract leaves of phyllanthus acidus was tested by Disc Diffusion Method. Standard antibiotic discs of Kanamycin (30μg/disc) for bacterial species were used as standard and crude extracts were used at a concentration of 400μg/disc and 500 μg/disc. The combination effect of phyllanthus acidus extract and drugs are more than single drug concentration. The combination therapy is extract+ciprofloxacin, extract+levofloxacin, extract+ceptriaxone, extract+cefuroxime, extract+azithromycin and extract+nystatin. The Antibiotics showed good result against gram positive, gram negative and resistant bacteria. Some antibiotics showed no result against bacteria such as Cefuroxime and Nystatin. But when they are combinedly used with extract of phyllanthus acidus they showed result against most of the gram positive, gram negative and resistant pathogen. When Nystatin with extract of phyllanthus acidus is given combinedly showed no result for the pathogenklebsiella spp(Resistant pathogen). But this combination therapy showed good result for the resistant strain of klebsiella spp and here the zone of inhibition is 22mm. Combination therapy of extract of phyllanthus acidus and cefuroxime showed no result for the pathogen salmonella typhi. But these combination therapy also give result for the resistant strain of Escherichia coli, and the zone of inhibition is 22mm. The effect of combination of phyllanthus acidus and azithromycin is more than azithromycin to against Shigella dysenteriae and Vibrio cholera.

Keywords: Antibiotics, Antibiotics resistant, phyllanthus acidus.