INVESTIGATION OF THE ANTIMICROBIAL ACTIVITIES OF Phyllanthus urinaria LEAVES BEFORE AND AFTER COMBINED WITH Pandanus tectorius FRUIT EXTRACTS
*Md. Ibrahim Sunny, Yesmin Begum, Md. Siddiqul Islam, Abdullah-Al-Mamun, Tarek Rahman
ABSTRACT
The study was to investigate the antimicrobial activities of Phyllanthus urinaria leaves extracts before and after combined with Pandanus tectorius fruit extracts. This was accomplished by examining the influence of the total aqueous, chloroform and ethylacetate extracts. The biological tests were done on Candida albicans, Salmonella typhi, Pseudomonas aeruginosa and Staphylococcus aureus. Agar diffusion test was applied to determine the antimicrobial activities. Based on the inhibition zone diameter, the result showed that Phyllanthus urinaria aqueous extraction had the inhibition on the Pseudomonas aeruginosa (22.75 ± 0.96mm) and Staphylococcus aureus (25.75 ± 0.96mm). The activities decreased after combined with Pandanus tectorius fruit extracts on Pseudomonas aeruginosa (14.75 ± 0.50 mm) and Staphylococcus aureus (15.75 ± 0.50 mm). However, the activities of these fractionated extracts in the combination showed the weaker activities than in the using only Phyllanthus urinaria extracts. The project was to investigate of the antimicrobial properties of Phyllanthus urinaria leaves extract and the combination of Phyllanthus urinaria with Pandanus tectorius extracts.
Keywords: Phyllanthus urinaria, Pandanus tectorius, Antimicrobial activity.
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