EFFECT OF WHITE AND BLUE LIGHT?EMITTING DIODE EXPOSURE ON Na+/K+?ATPase ACTIVITY IN HUMAN ERYTHROCYTE GHOST MEMBRANES
Mukunda Chethankumar*, Syeda Hajira Banu and Shamarao Nagashree
ABSTRACT
The present study analysed the effect of white and blue Light-Emitting Diode (LED) exposure on Na+/K+ATPase activity in isolated human erythrocyte ghost membranes. The effect of white and blue LED exposure was also studied on human blood hemolysis, calf thymus DNA and the cell viability after exposure was assessed using pollen grains. White and blue LED showed maximum hemolysis at 45 and 35min respectively in comparison to UV light, which was proportional to the exposure time. The decreased Na+/K+ATPase activity upon exposure of human erythrocyte ghost membranes to white and blue LED was restored at ATP concentrations of 2 mM, 3 mM, 4 mM and 5mM by 1, 1.5, 1.7 and 1.9 fold respectively. Fragmentation of calf thymus DNA was observed when exposed to the three types of light in the order UV > Blue LED > White LED. The cell viability was decreased by 50% in UV and blue LED light whereas white LED showed moderate cytotoxicity effect. Thus the study demonstrates that cell membranes are damaged and inturn affecting the DNA upon exposure to blue LED. This will possibly support the fact that blue LEDs are not favourable to normal cells when used in phototherapy to treat the diseases.
Keywords: Blue LED, white LED, cell viability, erythrocyte ghost membranes, hemolysis, Na+/K+?ATPases.
[Full Text Article]
[Download Certificate]
Impact Factor : 8.181 
