A COMPARATIVE IN VITRO CYTOTOXICITY STUDY OF COLLOIDAL SILVER AND CHITOSAN STABILIZED SILVER NANOPARTICLES ON MCF-7 AND HEPG2
P.Prema* and S.Thangapandiyan
ABSTRACT
Recently nanotechnology research was discussed for the generation of
nanoparticles as anticancerous agents. It is mandatory to elaborate the
application of colloidal silver and chitosan stabilized silver
nanoparticles in general and anticancerous property in particular. The
present study was aimed to investigate the in vitro cytotoxicity effect
of colloidal silver and chitosan stabilized silver nanoparticles against
human breast cancer cells (MCF-7) and liver cancer cells (HepG2)
towards the development of anticancer drugs. The colloidal silver and chitosan stabilized
silver nanoparticles were fabricated by using sodium borohydride as novel reducing agent. It
was well characterized by UV, SEM, EDS, XRD, and AFM studies showed spherical shaped
nanoparticles in the size range of 20-40nm and in slightly agglomerated form. Surprisingly it
also showed cytotoxic effect against MCF-7 and HepG2 cell lines were confirmed by MTT
and cell viability assays. There was an immediate induction of cellular damage in terms of
loss of cell membrane integrity, oxidative stress and apoptosis were found in the cell which
treated with colloidal silver and chitosan stabilized AgNPs. This may be a first report on anti-
MCF-7 and anti-HepG2 property of colloidal silver and chitosan stabilized AgNPs in the
fourth generation of nanoparticle research. It is necessary to study the formulation and
clinical trials to establish the nano drug to treat cancer cells.
Keywords: colloidal silver, chitosan stabilized AgNPs, MCF-7 and HepG2.
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