European Journal of Biomedical and Pharmaceutical Sciences

ANTI-HYPERGLYCAEMIC ACTIVITY OF LEAVES OF ANNONA RETICULATA LINN.ON EXPERIMENTAL DIABETICRATMODEL

Kalyani Pathak* and Aparoop Das

ABSTRACT

Objective: The present study has been designed to determine the effectiveness of five different extracts of leaves of Annona reticulata L. for potential hypoglycemic activity against streptozotocin (STZ) induced diabeticrats. Materials and methods: Hyperglycemia was induced in rats by administration of multipledoses (60 mg/kg) of Streptozotocin (STZ) to the overnight fasted rats for five consecutive days intraperitoneally. After 10 days of STZ administration, the fasting blood glucose levels (FBG) were measured and the rats with FBG level >200 mg/dL were considered to be diabetic and were used in the study. The study was undertaken by using the plant extracts at dose levels of 500 mg/kg (as test), p.o. and standard drug as Glibenclamide (4.5 mg/kg) in both normoglycemic and streptozotocin induced hyperglycemic rats. Anti-hyperglycemic activity was assessed by the measurement of fasting blood glucose, physical parameters (body weight, food and water intake) and Oral Glucose tolerance test. Results and discussion: Results of the studies showed that, the fasting blood glucose level in hyperglycemic and in oral glucose tolerance test, showed a significant (p<0.01) decrease at defined time points. After 21th days treatment the leaf methanol extract was found to be most potent at a dose of 500mg/kg. It brought down the elevated blood glucose level in streptozocin induced diabetic rats nearer to the normal range. Conclusion: The present study suggests that Annona reticulata Linn. leaves possess potent hypoglycaemic activity.

Keywords: Annona Reticulata, Streptozotocin, Glibenclamide, Hyperglycemia, Diabetic.