Abstract
CALIBRATION VERIFICATION FOR OLYMPUS AU 480 AND MERIL AUTOQUANT AQ 400i AUTOMATIC BIOCHEMISTRY AUTO ANALYSER USING SEVEN ANALYTES

Prof. Krishnajyoti Goswami*, Dr. Subrata Mookherjee and Dr. Ipsita Mazumdar

ABSTRACT

Background: Routine diagnostic laboratories are confronted with an ever-increasing workflow with limited resources. Automation has provided some solutions to these challenges, particularly with high throughput analysers such as the Meril AutoQuant AQ 400i. Objective: The objective of this study was to perform the analytical evaluation of the clinical chemistry analyser Meril AutoQuant AQ 400i, according to the guidelines of the European Committee of Clinical Laboratory Standards (ECCLS). Study design: The evaluation study determines within-run and between-run imprecision, inaccuracy in comparison with Olympus AU 480. The tested analytes were: glucose, albumin, creatinine, calcium, cholesterol, alkaline phosphatase and lactate dehydrogenase. Results: The result shows that both imprecision (CV%) and inaccuracy (Bias, B%) were < 5% in all parameters of both normal (Level 1) and pathological (Level 2) controls except for alkaline phosphatise (AlkP), where the CV% is slightly higher than 5% (5.37) in normal control (Level 1). Regression analysis studies reveal that the both Bias, (B%) and CV% are maintaining a mathematical relationship ( R2 = 1.0) either in positive or negative directions depending on nature of test parameters. Conclusion: The study describes the analytical performance of Meril AutoQuant AQ 400i, using seven routine chemistry parameters. Meril AutoQuant AQ 400i auto analyser shows acceptable precision and accuracy for majority of analytes. This evaluates the auto analyser as able to perform rapid and precise tests suitable for a fully automateanalytical procedure. Regression analysis also exhibits near equivalent data ranging from 0.9 to 1.1.

Keywords: Chemistry analyser, Analytical performance, Precision analysis, Regression analysis, Analytical evaluation.


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