European Journal of Biomedical and Pharmaceutical Sciences

SCREENING OF Tagetes minuta ETHANOLIC EXTRACT FOR ITS PHYTOCHEMICAL COMPOSITION, ANTIOXIDANT PROPERTY, TOTAL PHENOLIC AND TOTAL FLAVONOID CONTENTS

*Mir Ajaz Akram, Manju Tembhre, Muzafar Ahmad Sheikh, Shah Khalid, Feroz Ahmad Shergojri, Poonam Ahirwar

ABSTRACT

Medicinal plants are widely distributed in Jammu and Kashmir and their biological role and phytochemical composition are not still fully reconnoitered. The present work was undertaken to explore the preliminary phytochemical screening and antioxidant value of ethanolic extract of Tagetes minuta (EETM). The study was done by using various in vitro methods such as, Preliminary qualitative phytochemical assay, DPPH radical scavenging activity, reducing power assay and, Total phenolic and Total flavonoid content estimations. The whole EETM showed a fair number of phytochemicals with high free radical scavenging activity as evidenced by low IC50 in DPPH (40.77 μg/ml) and increase in absorbance in case of reducing power assay. The TPC and TFC of the extract were found to be was 130.80.6 mg/g Gallic acid equivalent and 57.332.51 mg/g Rutin equivalent of the extract respectively. The findings from the present investigation clearly showed that EETM possessed high phenolic and flavonoid contents and potential antioxidant activity and could be used as a valuable source of natural antioxidants and hence can prevent the body from diseases associated with oxidative stress.

Keywords: Tagetes minuta,Gallic acid, Rutin and Antioxidants.