PREVALENCE OF UROPATHOGENIC ESCHERICHIA COLI & STAPHYLOCOCCUS AUREUS AMONG PATIENTS WITH CASES OF URINARY TRACT INFECTIONS ATTENDING BENUE STATE UNIVERSITY TEACHING HOSPITAL, MAKURDI
Abbas Abel Anzaku*, Upla Peter1, Mfonido Ukpanah, Opedun Temitope O. A. and Odonye Dauda
ABSTRACT
In the present study, we investigated the prevalence of few bacterial organisms associated with urinary tracts
infection and their antimicrobial susceptibility patterns. Total of Ninety (90) midstream urine samples were
collected from outpatients with respect to their age group, gender and marital status attending health facility in
Benue State University Teaching Hospital Makurdi. Each 0.1ml of urine sample was cultured on MacConkey agar
and colonies showing lactose fermentation were subsequently subcultured on Eosin Methylene Blue Agar to
obtain a pure culture of E. coli whereas S. aureus was isolated by inoculating 0.1 ml of urine sample on Cysteine
Lactose Electron Deficient (CLED) agar and subsequently subcultured into Manitol Salt Agar to obtain pure
culture. One way analysis of variance (ANOVA) and paired t-test was carried out using IBM SPSS
STATISTICAL version 22. Based on the result obtained, 33(36.67%) prevalence of E. coli and S. aureus was
observed. E. coli has the highest occurrence of 19(95.00)% while S. aureus has 14(70.00%). There was significant
variation within the age group at ρ<0.05 with the highest 11.11% occurring in 27-32 years while the lowest was
4.44% in 33-38 and 39-44years respectively. On the gender basis, female had highest occurrence of 21.11% while
male has the lowest of 15.56%. Variation in the positive samples on marital status showed significant difference
with highest among married subjects 21.11% while the lowest occurred in single 15.56% at ρ<0.05. E. Coli was
sensitive to Ofloxacin (15.00mm), Ciprofloxacin (12.00mm) and Prefloxacin (12.22mm). and resistant to
amoxicilin and ampicilin while S. aureus was sensitive to amoxicilin (14.44), ofloxacion (12.22mm) and
ciprofloxacin (12.22mm) but resistant to vancomycin and prefloxacin respectively. Based on this study, many
isolates were resistant to the antimicrobial agents. This therefore recommended recommends antimicrobial
sensitivity assay to know the most effective drugs before administration of any drug for UTIs.
Keywords: Prevalence, E. coli, S. aureus, Outpatients, Urinary Tract Infection.
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