European Journal of Biomedical and Pharmaceutical Sciences

TOTAL ANTIOXIDANT CAPACITY OF LEAF, STEM, ROOT AND FLOWER OF DATURA METEL

R. Piratheepkumar* and R. Sivakanesan

ABSTRACT

In Srilanka it Datura metel is used in Ayurveda and Siddha treatment systems for treating bronchial asthma and chronic wound healing respectively. It is also used to maintain heath as a rejuvenating herb. The purpose of the study was to investigate the Total antioxidant capacity of the different parts of Datura metel plant. The plant was obtained from Government herbal garden, and taxonomically authenticated by officer In charge of medicinal plants division, Unit of Siddha Medicine, University of Jaffna. The collected plants were washed thoroughly with running tap water and separated into leaf, flower, stem, and root immediately. Separated parts were dried in sun shade. The dried parts were powdered and stored in air tight containers. Cold and hot water extract were prepared. Total antioxidant capacity (TAC) was measured by the ferric reducing antioxidant power expressed as μmol/g dry weight (DW). TAC values of cold water extract were 450.3 ± 31.99 to 647.2 ± 32.93 and values of hot water extracts were 499.1 ± 42.49 to 702.7 ± 45.15μmol/g DW. Datura metel leaf contained the highest amount of TAC in both hot and cold water extracts. TAC values were higher in hot water extract than cold water extract. Flower sample showed the lowest TAC. However all plant parts showed moderate level of Anti-oxidant capacity.

Keywords: Datura metel, Antioxidant.