European Journal of Biomedical and Pharmaceutical Sciences

OPTIMIZATION OF PHYSICAL ENVIRONMENT AND SOME NUTRIENTS FOR THE PRODUCTION OF L-LYSINE BY A MUTANT Micrococcus glutamicus SG300

Subadeep Ganguly and *Kunja Bihari Satapathy

ABSTRACT

L-lysine is an essential amino acid used as a feed supplement. It is produced by chemical, enzymatic and fermentation processes. In our present study, a mutant strain Micrococcus glutamicus SG300 developed in our laboratory, had been employed for optimization of different physlcal environmental conditions and some nutritional parameters especially carbon and nitrogen sources for L-lysine production. Initially, the strain accumulated only 6.2 g/ L of L-lysine in the fermentation broth. After optimization of different parameters, the production appreciably enhanced 17.8 g/L with pH, 7.5; temperature, 320C; fermentation time , 72h; volume of medium, 20ml; inoculums size, 10% with cell density 107 cells/ml; agitation, 200rpm by using biotin, 0.2μg/ml; glucose, 10% and urea, 10%. [Lysine, amino acid, Micrococcus glutamicus, physlcal environmental, nutritional parameters].

Keywords: Lysine, amino acid, Micrococcus glutamicus, physlcal environmental, nutritional parameters.