European Journal of Biomedical and Pharmaceutical Sciences

METHYL ACETATE FRACTION OF METHANOLIC EXTRACT OF SCHREBERA SWIETENIOIDES ROXC. ROOT FOR IT’S ANTIOXIDANT, ANTI-INFLAMMATORY AND ANTIPYRETIC ACTIVITIES

Syed Wajid and *Taqui Mohammed

ABSTRACT

Ethanolic extract of Schrebera swietenioides roxb. (SSE) root was prepared by soxhlation method and screened for its in vitro antioxidant, anti-inflammatory (acute, chronic) and antipyretic activity in wistar rats. Antioxidant assay was performed by using DPPH, ABTS and Lipid peroxidation assay methods. In antioxidant assay, % inhibition and IC50 was calculated. SSE produced 50% inhibition (IC50) at 1.7 μg in DPPH, 4.35μg in ABTS, 23.53μg in lipid peroxidation assay and shown more potent antioxidant activity compare to standard compound. Anti-inflammatory activity was performed by using carrageenan induced paw edema and complete Freund’s adjuvant arthritis in rats. In these assays, SSE showed dose dependant inhibition in paw edema. Maximum inhibition was found at 300 mg /kg oral dose of SSE in rats. In Carrageenan induced paw edema SSE showed maximum 67.81% inhibition and in freund’s adjuvant arthritis it showed maximum 50% inhibition at 300 mg/kg oral dose in rats, which was significantly different from control (p<0.05). SSE also showed dose dependant decrease in body temperature in brewer’s yeast induced pyrexia in rats. SSE significantly decrease in body temperature (p<0.05) at 300 mg/kg compare to control. Present study showed that SSE had significant antioxidant, anti-inflammatory and antipyretic activity.

Keywords: Antioxidant, anti-inflammatory, antipyretic, Schrebera swietenioides roxb., DPPH, ABTS.