European Journal of Biomedical and Pharmaceutical Sciences

IN VITRO HIV-1 REVERSE TRANSCRIPTASE INHIBITION OF ANDROGRAPHOLIDE ISOLATED FROM ANDROGRAPHIS PANICULATA

Swapna Gurrapu and Estari Mamidala*

ABSTRACT

HIV is a major global concern due to its drug resistance and warrants the development of novel antivirals as alternative therapy. In the current study, andrographolide was isolated from Andrographis paniculata and HIV-1 reverse transcriptase inhibition was assessed. The crude extracts were prepared from dried areal parts Androgaphis paniculata in ethanol by maceration method and isolated andrographolide by using column chromatography and HPLC. Peripheral Blood Mononuclear Cells (PBMCs) isolated from healthy donors by ficoll–hypaque density gradient centrifugation method. Cell viability test was performed on andrographolide by MTT assay against PBMC and HIV-1 RT inhibition activity was determined by HIV-1 Reverse Transcriptase (p66) Capture ELISA. In the HIV-1 reverse transcriptase assay, isolated compound showed good inhibitory activity (87%) at 160 μg/ml and MTT assay confirmed that the isolated andrographolide had no cytotoxic activity and IC50 values higher than 100 μg/ml.

Keywords: HIV, Andrographis paniculata, reverse transcriptase, MTT assay.