Abstract
OPTIMIZATION PARAMETERS FOR PRODUCTION OF ?-AMYLASE BY BACILLUS MEGATERIUM KLMI4

Lingappa K.* and Masarath Irfana

ABSTRACT

The present study reports on the optimization of process parameters for the production of α-amylase through submerged fermentation (SmF) by a bacterium, Bacillus megaterium KLMI4, in mineral medium amended with soluble starch. The organism was isolated from a cultivated arid soil of Kalaburagi district in the northern part of Karnataka State. The optimum levels of the parameters were observed to be: pH 8.0, temperature 370 C, inoculum size of 3x106 cfu/ml per 100 ml mineral medium and substrate concentration of 1.5% soluble starch. The crude α-amylase was stable in the pH range of 7.0 to 9.5 and temperature range of 310 to 450 C. After optimizing the parameters, α-amylase production was observed to be enhanced by almost 6 times over that observed before optimization.

Keywords: ?-Amylase, starch hydrolysis, B. megaterium KLMI4, process optimization, submerged fermentation.


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