European Journal of Biomedical and Pharmaceutical Sciences

DEVELOPMENT OF REAL TIME PCR ASSAY FOR EARLY DETECTION OF CYTOMEGALOVIRUS INFECTION IN IRAQI WOMEN

Rehab S. Ramadhan* and Sara A. Jihad

ABSTRACT

This study was constructed to discuss a Molecular Study of Cytomegalovirus isolated from women with repeated miscarriage in relation to immune response molecule Tall like Receptor2. About (100) blood samples from women suffering from infection with Cytomegalovirus were collected from infertility clinic of Kamal Al –Sammaraee hospital and (50) samples from normal subjects served as control for comparison. Test subjects were divided into two age groups: 20-30 years old and 31-40 years old. The women distributed as (60) samples of infertile and (40) samples as miscarriage women. This study included Enzyme Linked Immune Sorbent Assay (ELISA) test was used to detect anti- HCMV antibodies IgG and IgM in the patient serum samples. ELISA test result showed that the miscarriage women shown highest percentage of seropositive to CMV for IgG (40%) and (25%) IgM compared to infertile women IgG (20%) and (15%) IgM with a significant difference P<0.05. It has been concluded that there is high prevalence of anti- HCMV antibodies IgG and IgM in both miscarriage and infertile women compared with normal (healthy) women in Baghdad, The Seropositive of anti- HCMV IgG was higher in younger women (20-30) years old while the largest age classes (31-40) years showed higher level of IgM. The CMV primers were selected from highly conserved region of the major enveloped glycoprotein B (gB) and used probe labeled at the 5 end with FAM and the 3 end with TAMRA. The result shown amplification from the sixth cycle.

Keywords: Cytomegalovirus, miscarriage, infertile, ELASA, RT-PCR.