European Journal of Biomedical and Pharmaceutical Sciences

DEVELOPMENT AND VLAIDATION OF SIMPLE UV-SPECTROPHOTOMETRIC METHOD OF QUANTIZATION OF DOMPERIDONE IN SOLID DOSAGE FORMULATION USING MIXED SOLVENCY CONCEPT

Sanjay Jain*, R. K. Maheshwari, Rajesh Kumar Nema and Indrajeet Singhvi

ABSTRACT

Organic solvents are most frequently employed in spectrophotometric analyses. They may be sources of pollution. Some of them may be toxic while others may be costlier. Volatility may be a source of inaccuracy in spectrophotometric estimations. In the present investigation, it was proposed to solubilize Domperidone by use of mixed solvency concept. Domperidone shows maximum absorbance in the concentration range of 10-50μg/ml at 282 nm. Method of analyses have been validated for different parameters like linearity, accuracy, precision, LOD and LOQ. The percent drug estimated in tablet formulation of Batch-I and of Batch-II were 101.12±1.248 and 100.54±2.100 respectively. The range of percent recoveries varied from 100.62±0.308 to 101.70±0.151.Urea and sodium Caprylate do not interfere above245nm. The analytical method was found to be simple, safe (free from toxicity), economic and eco-friendly.

Keywords: Domperidone, UV-Spectrophotometry, Solid Dosage Formulation, Mixed Solvency Concept.