European Journal of Biomedical and Pharmaceutical Sciences

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF CEFDINIR IN BULK AND PHARMACEUTICAL DOSAGE FORM BY UV-VISIBLE SPECTROPHOTOMETRIC METHOD

Krishnaphanisri Ponnekanti and Raja Sundararajan*

ABSTRACT

The objective of the study was to develop and validate easy, accurate and precise UV- Visible spectrophotometric method for the determination of cefdinir in bulk and pharmaceutical dosage form. Spectroscopic method development for the estimation of cefdinir was carried out using Shimadzu 1800 UV Visible Spectrophotometer with a pair of 10mm path length matched quartz cells. All the solutions were scanned in the range of 200-400 nm with medium scanning speed. Zero order (Method A) and First-order derivative (Method B) spectrophotometric methods have been developed for the determination of cefdinir in 0.1N hydrochloric (HCl) acid solution. The result of the study, absorbance of cefidnir was measured at 281nm for the zero order. Zero order spectra were derivatized into first order. Beer’s law was obeyed in the range of 1-55μg.ml. The methods were validated with respect to linearity, accuracy, precision and LOD & LOQ. The linearity concentration range was 1–55μg/ml and the correlation coefficients (r2) were found to be > 0.999. The mean % recoveries were found to be in the range of 98.5%–101%. The percent relative standard deviation (RSD %) of inter day precision range was found between 0.23 – 1.15% and intraday precision range was found between 0.35 – 0.81%. It can be concluded that the proposed method was validated by determining its accuracy, precision and sensitivity which proves the suitability of the method for the routine estimation of cefdinir in bulk and solid dosage form.

Keywords: Cefdinir, UV Visible spectrophotometer, Zero order, First-order derivative.