PRELIMINARY PHYTOCHEMICAL, UV-VISIBLE, FTIR AND INVITRO ANTIOXIDANT STUDIES OF AQUEOUS METHANOLIC EXTRACT OF CANTHIUM PARVIFLORUM STEM BARK (RUBIACEAE)
Dr. Venu Sampath Kumar Golla* and Ravichandra Sharabu
ABSTRACT
Objective: The aim is to investigate the preliminary phyto chemical screening, UV-Vis, FTIR analytical profile and in vitro anti oxidant studies of aqueous methanolic extract of Canthium parviflorum stem bark. Methods: Preliminary phyto chemical screening is carried out by standard procedures. The extracts were tested against free radicals by using DPPH method comparing to standard. Results: The results showed the presence of flavonoids, phenolic groups at 360nm, 214nm and their maximum absorbance values (0.078cm-1, 1.392cm-1) respectively and their functional groups are detected at what peaks (3315.03cm-1, 1523.49cm-1, 1715.37cm-1, 1617.98cm-1, 1159.97cm-1) they got identified and these were tabulated. Free radical scavenging activity of aq. Methanolic extract of canthium parviflorum stem bark is IC50=400μg/ml (50.89%) comparing with the standard drug is IC50=6.0μg/ml (58.67%) only. Conclusion: the performed analytical studies signified the presence of various phytochemical like phenols and flavonoids. Further studies determined the antioxidant study by using DPPH through in-vitro method. The outcome of these studies stands to be a platform for future conducting a choice of various pharmacological studies to make out the potency of these extracts.
Keywords: UV-Visible analysis, FTIR, Canthium Parviflorum, DPPH, Anti-oxidant.
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