European Journal of Biomedical and Pharmaceutical Sciences

THIAMINASE ACTIVITY OF THIAMINOLYTICUS CULTURE IN PRESENCE OF DIFFERENT FORMS OF THIAMINE WITH OR WITHOUT VITAMIN C

*Sarwar M and Mazin O. Mohager

ABSTRACT

Vitamin B1 also called Thiamine when deficient caused Beri Beri and effects a large proportion of world population especially in third world countries. In Europe, North America and Australia, thiamine deficiency is common among alcoholics and usually manifests itself as the Wernicke-Korsakoff syndrome but has also been reported in patients on restricted diets for obesity, those who receive total parenteral nutrition and in those who are on fad diets or whose intakes are high in carbohydrate and low in thiamine. Thiaminase I is a transferase-type enzyme that catalyzes the displacement of the thiazole moiety of thiamine (vitamin B1) by a wide variety of nucleophiles: aromatic primary amines, heterocyclic compounds, and sulfhydryl compounds (i.e., co-substrates). Thiaminase activity occurs among cyanobacteria), plants, bacteria invertebrates, shellfish, and fish. Other enzyme, thiaminase II, that catalyzes the hydrolysis of thiamine occurs exclusively in microorganisms. While the thiaminase II in microorganisms seems to be involved in the thiamine salvage pathway the physiological significance of thiaminase I in species that contain the enzyme is not known and consequently there is a lack of knowledge concerning the possible agents or factors that cause the wide variation in thiaminase activity observed within organisms. The presence of thiaminase in the diet has been associated with the occurrence of thiamine deficiency symptoms among mammals, including humans. In present work it was in mind that in pure culture of thiaminolyticus effect of added thiamine in its various biological forms should be tested at different time intervals of the growing culture with or without the addition of vitamin C. It is revealed that maximum activity of the thiaminases of thiaminolyticus culture is found in 24 hours culture in presence of thiamine hydrochloride. Interestingly when Vitamin C is added it enhanced 13% more activity in thiamine pyrophosphate containing culture. Addition of Vitamin C also enhance 33% SOD activity in the culture containing thiamine pyrophosphate. Catalase activity was found to be increased from 5% to 9.1% in different forms of thiamine containing cultures. The addition of Vitamin C enhanced glutathion peroxidase activity from 8.5% to 13 %. It is therefore, concluded that addition of vitamin C can increase the thiaminases and anti oxidant enzymes activities in thiaminolyticus cultures and hereby indicates some underlying mechanism which needs to be explored in future. We also concluded that Vitamin C can be be beneficial in the deficiency of thiamine especially if it is caused by thiaminases through its antioxidant profile.

Keywords: Antioxidant enzyme, Paenibacillus thiaminolyticus, vitamin C, thiamine, thiamine pyrophosphate.