THE ROLE OF INTERLEUKIN-10 PROMOTER POLYMORPHISM RS1800872 (-592 C>A) IN SUDANESE HEPATITIS B INFECTED PATIENTS
Amna Abu Elgasim Abd-Elrhman Bedri*, Dr. Sahar Mubarak Bakhiet and Dr. Mona Omer Awad A. Elkareem
ABSTRACT
Hepatitis B virus (HBV) infection is a serious global public health problem. The outcomes of hepatitis infection is determining between viral factors and host genetic as Interleukin-10 (IL-10) which is a multifunctional cytokine participates in the susceptibility of many diseases., The polymorphisms in promoter region are more important than others, which regulate gene transcription. Objectives: Aimed to evaluate the correlation between HBV infection and polymorphisms (SNP) in IL-10 gene polymorphisms (-592C/A.) Material and methods:- Hepatitis B virus (HBV) infection In Sudan as in other developing countries there are increases, this study was performed on 46 cases of Hepatitis B and 48 healthy matched controls. DNA was extracted and PCR for genotyping of IL10 promoter polymorphism -592C/A was applied followed by restriction fragment length polymorphism (RFLP). In this study participants age ranged from 17 to 67 with Mean (SD) [34.1±15.1] years (male 85, female 10). Results: - Three genotypes were detected Using PCR-RFLP method CC for wild type, AC heterozygous and AA homozygous mutant. Wild type CC was more frequent among controls 72%. Allele C frequency was higher in controls group when compared to allele A which increased among patient's. All genotypes and allele frequency were in Hardy Weinberg Equilibrium (HWE) among cases. Single point analysis using the fisher test and chi square showed significant Correlation between -592 promoter polymorphisms and the disease with p. value 0.01. These results suggested that IL-10 592 C/A (rs 1800872) polymorphism appears to have some influences on the infection of HBV
Keywords: rs1800872, Promoter polymorphism, IL- 10, Hepatitis B virus
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