European Journal of Biomedical and Pharmaceutical Sciences

BIODEGRADATION OF PHENOLIC MIXTURES AT HIGH INITIAL CONCENTRATIONS BY TRAMETES VERSICOLOR 1 IN A “FED-BATCH” PROCESS

Galena Angelova*, Ivanka Stoilova, Kolyo Dinkov and Albert Krastanov

ABSTRACT

Biodegradation of mixed substrates phenol and catechol and phenol and 2,4-dichlorophenol at high inhibitory initial concentrations (2 g/dm3) by Trametes versicolor 1 in a “fed-batch” process is studied. It is established that Trametes versicolor 1 (biomass 50 g/dm3) biodegrades total 6.14 g mixture of phenol and catechol for 14 d at 2 feedings and 2.57 g mixture of phenol and 2,4-dichlorophenol for 8 d at 1 feeding. It is found that Trametes versicolor 1 synthesizes phenol-hydroxylase, catechol 1,2-oxygenase – enzymes, required for biodegradation of substrates in the two studied phenolic mixtures. Laccase is produced in media containing substrates phenol and catechol, in the media comprised mixture of phenol and 2,4-dichlorophenol this enzyme is not being synthesized. The lysis of Trametes versicolor 1 during the biodegradation of phenol and catechol (79,2 %) and phenol and 2,4-dichlorphenol (76,4 % ) is weaker than the lysis during the biodegradation of phenol (96 %) at the same initial concentration (2 g/dm3).

Keywords: Trametes versicolor, biodegradation, phenol, 2,4-dichlorophenol, catechol, phenol hydroxylase, catechol 1,2-oxygenase, laccase.