BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF ACTINOBACTERIA ANTAGONISTIC TO CLINICAL PATHOGENS FROM SOUTHEASTERN ARABIAN SEA MARINE SEDIMENT
Pandurangan Ayyanar and Natesan Manoharan*
ABSTRACT
Streptomyces are common soil bacteria that produce secondary metabolites, including several antibiotics; however, the characteristics of marine Streptomyces are largely unknown. Hence marine sediments, collected from the coastal areas from off Cochin fort (Kerala) latitude and longitude (9°57’52.8” N, 76°14’05.1” E). Southeastern Arabian Sea, India. Totally twenty isolates were obtained among this isolatesone isolate namely INR1 showed significant antibacterial activity against both clinical and fish pathogens. Morphological, cultural and biochemical characterization indicated that the isolate belong to Streptomyces genus.Molecular characterization of INR1 was evaluated by PCR amplification of 16SrRNA gene. Phylogenetic analysis of16S rRNA sequencing studies revealed that the strain is closely related to Streptomyces mutabilis. Further investigations may yield greater understanding of these organisms in this and other marine environments and may be a reservoir of novel microorganisms and secondary metabolites.
Keywords: Streptomyces mutabilis INR1, antimicrobial activity, 16S rRNA sequences, Microbial pathogens, phylogenetic analysis. Sediment samples were taken from 3 sites in the Laguna Madre to isolate marine Streptomyces. Diverse characteristics with regards to their
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