European Journal of Biomedical and Pharmaceutical Sciences

HIGH FREQUENCY CALLUS INDUCTION FROM SHOOT BASE EXPLANTS OF ALOE VERA (L.) BURM.F AN IMPORTANT MEDICINAL PLANT

Rajendra Prasad B.*, Rajesham P., Venkateshwarlu M. and Nagaraju M.

ABSTRACT

The present study aimed to evaluate the callus induction from the shoot base derived calli of Aloe vera was described. The shoot base explants were excised from in vivo plantlets cultured on Murashige and Skoog (MS) medium supplemented with 2, 4-dichloro phenoxy acetic acid 2, 4-D/IAA (1.0 - 3.0 mg/L). After one week the initiated cell debris was transferred on MS medium fortified with IAA (2.5 mg/L). The sub cultured cell debris gave rise to luxuriantly growing calli after two weeks. These calli were sub cultured on MS medium supplemented with various concentrations of IAA (2.0 - 30 mg/L) alone and with NAA (1.0 - 5.0 mg/L) for efficiency callus induction. The efficient callus was observed on full strength MS medium supplemented with 2, 4-D (2.5 mg/L) along with NAA (2.0 mg/L) in three weeks of time.

Keywords: Aloe vera; Murashige and Skoog medium; Callogenesis; Shoot buds.