European Journal of Biomedical and Pharmaceutical Sciences

MASS PROPAGATION OF ACACIA MANGIUM THROUGH PLANT TISSUE CULTURE

Sonal Shukla and Dr. Sao Shweta*

ABSTRACT

The present study on “Mass Propagation of Acacia mangium Through Pant Tissue Culture” was conducted at Dr. C.V. Raman University Kargi road Kota, Bilaspur (C.G.). The man objective of plant tissue culture of mangium initiation was to standardize protocol for mass multiplication of Acacia mangium. Healthy explants were taken from the selected elite mother plant’s from the selected elite mother plant from mngium field and dipped in the 2% hgcl2 for 10 min. the initiation of micro propagation of Acacia mngium encircles Media preparation which includes MS Medium containing known concentration of the media. Shooting for shoot proliferation and multiplication SM = II Media. i.e. MS salt supple meted with 2 mg/lit. BAP kinetin and 25mg/lit. adenine sulphate. Was found to be best compared to other media prepared through number of multiple shoot’s were more in SM-II media but was not appropriate for subcultoring. Rooting Best rooting obtained in RM-II Media i.e.1/2. Strength MS- media supplemented with 2 mg/lit. NAA. Hardening og tissue culture growth plantlets Tissue culture of raised plantlet’s were subjected to specified culture culture regimes aimed at making them. Capable of surviving the uncontrolled and narshar ere vitro environment’sthis was cultured rised plantlet’s were hardened in to coco pet’s info the green house.

Keywords: Acacia mangium, Media, BAP, TAA, Hgcl2, NAA, EDTA.