European Journal of Biomedical and Pharmaceutical Sciences

KINETIC STUDY FOR EX-VIVO INTESTINAL GLUCOSE UPTAKE ACTIVITY OF METHANOLIC EXTRACT OF CANNA INDICA FLOWER BY MODIFIED EVERTED GUT SAC TECHNIQUE

Ch. Aruna Kumar* and S. Vidyadhara

ABSTRACT

Canna indica flower are traditionally used for the treatment of diabetes mellitus. Now a days everted rat gut sac technique was used to investigate the D-glucose uptake activity. In current study, modified Everted gut sac technique was performed with chick ileum instead of rat ileum. In this study the effect of methanolic extract of canna indica flower on glucose uptake by Everted guts was studied. The guts were mounted in a gut sac bath and different concentrations of glucose were added into mucosal fluid. Just before starting the experiment, the methanolic extract of canna indica flower (2mg/ml) was also added in the same compartment. After one hour of incubation, the glucose concentration in mucosal fluid and serosal fluid was measured by using photoelectric colorimeter. Michaelis-Menten constant (Km) and maximal velocity (Vmax) were calculated in the presence and absence of the flower extract. It was observed that methanolic extract of canna indica flower significantly increases the D – glucose uptake by 0.11 mM/hr.

Keywords: Everted gut sac, canna indica flower, Mucosal fluid, Chick ileum, D-Glucose.