COMPARATIVE STUDIES ON BIOCHEMICAL, HISTOPATHOLGICAL AND FTIR-ATR SPECTRAL VARIATIONS OF GENTAMYCIN INDUCED RENAL DISEASE IN WISTAR RAT
Saira Khatheeja*, Prabhakaran A. R., Sukumar E. and Safiullah A.
ABSTRACT
Establishment of FTIR-ATR spectroscopic method in disease diagnosis was attempted with induction of renal disease status in wistar rat with gentamycin. The routine methods were used to analyse the biochemical variations to determine the renal disease status via colorimetric, ELISA and Histopatholgically in addition to FTIR-ATR spectral analysis. To achieve this, experiments were conducted with wistar rat injected with gentamycin for 30 days. At the experiment termination blood serum and organs were subjected to rule out the bio markers towards development of renal disease. The spectral bands (4000 – 450 cm-1) obtained from serum as well as to different organs was characterized for biomarkers to assess the diseases status which aid in complete screening to control and management of the disease. The chemical bonding stretches reveals the nature of bio molecule levels in serum and different organs. The results shows that the peak ratio for amide I / glucose-str. (I1634/I1076) as well as amide II / ribose–phospholipid (I1538/I934) were significantly increased in gentamycin induced renal than control male Wistar rat with renal disease. The absorbance for internal peak ratio of blood serum for amide III erythrocyte / (cystic acid) s-s-str. (I1313 /I532) is highly significant among gentamycin induced. These results also support fatty change in liver due to hypoxia and persisting fatty change in liver, inflammation in kidney, interstitial inflammation and granulomatous reaction as well as increased urea and creatinine levels in blood. FTIR-ATR spectral analysis in may be suggested addition to other routine methods. FTIR-ATR infra-red spectrum provides information about biochemical components in blood as well as in different tissues are discussed
Keywords: Wistar rat, Renal disease, induction, gentamycin, FTIR-ATR spectral analysis.
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