THE ROLE OF ANTI-INFLAMMATORY INTERLEUKINE-4(-590C>T) AND PRO-INFLAMMATORY INTERLEUKINE-6(-174G>C) GENES POLYMORPHISMS WITH TYPE 2 DIABETES MELLITUS IN IRAQI PATIENTS
Miriam Jasim Shehab?, Ismail A. Abdul-Hassan and Mohammed Mahdi Al-Zubaidi
ABSTRACT
Type 2 diabetes mellitus (T2DM) is a common health problem which has reached epidemic proportions due to the rapidly increasing rates of this disease worldwide. Unbalance between pro- and anti- inflammatory cytokines cause different diseases like Type 2 diabetes mellitus. This study aimed to verify the association of IL-4 (-590C>T) and IL-6 (-174G>C) genes polymorphisms with type 2 diabetes mellitus incidence in Iraqi population. Peripheral blood samples were collected from 50 diabetic patients and 50 apparently healthy individuals from both genders. DNA was extracted and Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) were carried out to detect polymorphism at the -590 and -174 position of IL-4 and IL-6 genes and determined the genotypes for Iraqi population. The results revealed that only CC genotype was found in all samples studied respect to IL-4 (-590C>T) polymorphism. the GG genotype in diabetic was significantly (p˂0.05) lower than control group (64% versus 74%, respectively) while the GC genotype in diabetic was significantly (p˂0.05) higher than control group (32% versus 22%, respectively), and there was no significant difference between diabetic and control group in the CC genotype with a non significant differences in either G or C allelic frequencies between diabetic patients and control group for IL-6 (-174G>C) polymorphism. The results of the present study indicate that heterozygous GC genotype was associated with the incidence of T2DM.
Keywords: T2DM, Cytokines, Genes Polymorphisms, PCR-RFLP.
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