European Journal of Biomedical and Pharmaceutical Sciences

CHEMICAL AND BIOLOGICAL STUDIES OF SOME NEW HETEROCYCLIC SCHIFF BASE METAL COMPLEXES

Vinay K. Srivastava*

ABSTRACT

Metal Complexes of Schiff base have played a central role in the development of coordination chemistry. Various Schiff Base Complexes have been widely studied because they have antimicrobial, anticancer, analgesic, anti-inflammatory and herbicidal applications.[1,5] The Present investigation deals with the synthesis, characterization, antibacterial and cytotoxicity studies of the Schiff Base ligand 2-furylglyoxal-2'-aminothiophenol and its Co (II) and Ni(II) metal chelates. Most of the chemicals used were of AR grade. Laboratory grade chemicals, whenever used, were purified by standard methods, while the solvents were purified and double distilled before use. The ligand was prepared by the reported procedure.[6,7] C, H, N were analysed on Carlo-Erba micro analyzer model 1106. Metal contents were estimated by standard procedure. FTIR were recorded on Thermo Nicolet Avater 370. Electronic Spectra on a Shimadzu UV – 160A and spectronic-20 spectrophotometer. 1H and 13C NMR Spectra in dimethyl sulfoxide (DMSO) were recorded on Brucker WH 300 (200 MHz) and varian Gemini (200 MHz) Spectrometers using tetramethylsilane (TMS) as an internal reference. The conductance measurements were Carried out on a Model CM-180 Elico digital conductivity meter. Magnetic studies was done by a Guoy balance using Hg [Co (SCN)4] as the calibrant. Antibacterial activity was determined by the paper disc plate method. In addition the synthesized metal chelates have been evaluated for their cytotoxic activity against a Panel of different human cancer cell lines by using MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) assay. Metal Chelates of Co(II) and Ni(II) were synthesized by the reaction of ethanolic solution of the Schiff base ligand (FGATP) (2m mol) into the Ethanolic solution of Co(II) / Ni(II) Acetate (1m mol) for 2 hrs. The complexes obtained were filtered, washed with ethanol and dired (yield 70-79%) All the metal chelates were coloured, non hygroscopic in nature and stable at room temp. They were insoluble in common organic solvent but soluble in DMF And DMSO. The molar conductance values indicates their Non Electrolytic nature.

Keywords: Schiff Base, Spectral, antibacterial, cytotoxicity.