European Journal of Biomedical and Pharmaceutical Sciences

EFFECT OF COCONUT (COSCOS NUCIFERA L.) WATER ON THE PHARMACOKINETIC PARAMETERS OF IBUPROFEN IN RABBITS.

Ezealisiji M. Kenneth*, Abayomi E. Omotosho and Itokem K.

ABSTRACT

To evaluate the effect of pre and post administration of coconut water on the pharmacokinetic parameters of single dose ibuprofen (2.5kg/kg body weight) in rabbits. A total of nine male and female rabbits were used in this study. The single dose pharmacokinetics of ibuprofen (2.5kg/kg body weight) was evaluated before and after 30minutes of coconut water administration. Plasma ibuprofen concentrations were determined using precise, reversed-phase HPLC protocol. Model independent methods were used to evaluate the pharmacokinetics of ibuprofen. Data were analyzed by 2 way ANOVA testing followed by students’ t-test. The relative bioavailability was observed to be 62.149% and 69.250%. The result showed that there was a significant reduction in the bioavailability of the study group II and I when compared with the control group. It was also observed that the mean plasma concentration (Cmax) for the study group I and II (30minutes pre and post administration of coconut water) was found to be 12.3±1.20 and 14.3±1.40 respectively compared to the Cmax value of 17.9±1.60 for the control group with ibuprofen alone. This difference was considered quite significant when compared with those of the control group with ibuprofen alone administered (P < 0.05). The area under the mean plasma concentration versus time curve (AUCt-12) for the study group I and II was observed to be 3158.4 and 3591.3 μg/hr/ml respectively when compared to those of the control group (5082.0). there was a remarkable significant difference between the observed AUC1-12hr for the different study groups when compared with the standard but there was no significant increase in Tmax amongst the various groups when compared to those of the control, the Tmax were found to be 1.5±0.02 and 1.5±0.01 respectively for study groups I, II and control. The HPLC method adapted was validated by using various methods of validation parameters.

Keywords: Ibuprofen, bioavailability, plasma, pharmacokinetics.