European Journal of Biomedical and Pharmaceutical Sciences

CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY OF NEWLY SYNTHESIZED HETEROCYCLIC COMPOUND

M. Sathyanarayanan, M. Seeni Mubarak, S. Mohamed Rabeek, M. Syed Ali, V. Anuradha and N.
Yogananth

ABSTRACT

Present work comprises of synthesis of 3-Benzyl-2-6 bis-(3-nitrophenyl)piperidin-4-one and 3-Benzyl-2-6-bis(2- chlorophenyl)piperidin-4-one. The structure of the synthesized compound was elucidated by spectral studies such as IR, 1 H, 13C-NMR, Elemental analysis and Biological studies. The antibacterial activity of these derivatives was assessed with series of gram-positive and gram-negative bacteria. The antibacterial activity and the minimum inhibitory concentration (MIC) of the 3-Benzyl-2-6 bis-(3-nitrophenyl)piperidin-4-one and 3-Benzyl-2-6-bis(2- chlorophenyl)piperidin-4-one were carried out using agar disc diffusion with various concentrations of 25μg/ml, 50 μg/ml, 75 μg/ml and 100 μg/ml which results in inhibiting DNA synthesis by affecting the activity of DNA topoisomerase. The minimum antibacterial activity showed (T1) 6±0.36 mm and (T2) 5±1.21 mm diameter against E.coil in 75 μg/ml. MIC result of the both compounds 3-Benzyl-2-6-bis-(3-nitrophenyl)piperidin-4-one and 3- Benzyl-2-6-bis(2-chlorophenyl)piperidin-4-one revealed that, the activity was observed between 128-512 μg/ml concentration against Staphylococcus aureus and Bacillus subtilis.

Keywords: Piperidine, FT-IR, 1H, 13C-NMR and antibacterial activity.