ISOLATION OF SOIL FUNGI AND SCREENING OF IN VITRO ANTAGONISTIC POTENTIALITY OF TRICHODERMA VIRIDE AGAINST PENICILLIUM CHRYSOGENUM
Pintu Karmakar*, Payel Debbarma, Panna Das and Ajay Krishna Saha
ABSTRACT
The investigation documented total of 8 fungal inhabitants from the paddy field at Suryamaninagar, Tripura during
October 2016 to April 2017. Among them six fungi were identified as Aspergillus flavus, Penicillium
chrysogenum, Fusarium oxysporum, Cladosporium cladosporioides, Trichoderma viride and Curvularia lunata,
two unidentified and several sterile mycelia. Seasonal variation and percentage of contribution were analysed and
calculated. Among the identified mycoflora except Trichoderma viride all were reported to cause infection in
living plant tissue or storage tissue. Trichoderma viride was selected as potent bio control agent to test its
influence on the growth of pathogenic fungi Penicillium chrysogenum which is second dominant isolated fungus
in this study. The result of dual culture of Trichoderma viride and Penicillium chrysogenum showed the occurence
of 55.67% inhibition of mycelial growth of Penicillium chrysogenum. After 7 days onwards it was found that the
hyphal tips of Penicillium chrysogenum become disassociated.
Keywords: Mycoflora, Mycelial growth, Paddy field, Antagonistic activity, Dual culture.
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