European Journal of Biomedical and Pharmaceutical Sciences

ACUTE TOXICITY STUDY OF RASAYANA YOGA- AN OPTHALMIC POLYHERBOMINERAL FORMULATION IN EXPERIMENTAL RATS

V. K. Archana*, D. B. Vaghela and Mukesh Nariya

ABSTRACT

Rasayana Yoga is an experience based compound herbomineral Ayurvedic formulation, administered orally in the management of Primary open angle glaucoma in Shalakyatantra Outpatient department of Institute for Post Graduate Teaching and Research in Ayurveda. It consists of herbomineral drugs like Rasayana Churna (combination of Guduchi, Gokshura and Amalaki), Saptamrita Lauha (classical formulation containing Hareetaki, Vibheetaki, Amalaki, Yashtimadhu and Lauha Bhasma), Abhraka Bhasma and Swarnamakshika Bhasma. All these, herbal and mineral drugs are extensively used in traditional system of medicine and by folk practitioners in India, because of their remarkable therapeutic potential. The aim of the present study was to evaluate acute toxicity of the formulation in female wistar rats. Acute toxicity test was evaluated as per the Organisation for Economic Co-operation and Development - 425 guidelines with 2000 mg/kg as limit test. The aqueous suspension of the powdered drug was administered once orally as a single dose of 2000mg/kg of body weight to female wistar rats in sequential manner. The general behaviors of animals were continuously monitored for 1 hour after dosing and periodically for 24 hours. The animals were also monitored for any mortality during the first 24 hours and daily thereafter for a total of 14 days. Acute toxicity test results showed that Rasayana churna at 2000 mg/kg did not produce any behavioral and physiological changes and observable toxic effects during entire duration of study and all animals survived for 14 days of observation.

Keywords: Acute toxicity, Mortality, Primary open angle glaucoma, Rasayana Yoga.