European Journal of Biomedical and Pharmaceutical Sciences

RATIO SUBTRACTION, BIVARIATE AND AREA UNDER CURVE SPECTROPHOTOMETRIC METHODS FOR DETERMINATION OF ATENOLOL IN BINARY MIXTURE WITH INDAPAMIDE

Khalid Abdel-Salam Attia, Nasr M. El-Abasawi, Sherif Ramzy*

ABSTRACT

Three simple, accurate and precise spectrophotometric methods are used for determination of Atenolol (ATN) in a binary mixture with indapamide (IND) without prior separation namely; ratio subtraction method (RSM), bivariate and area under curve (AUC). The accuracy, precision and linearity ranges of the proposed methods were determined. The methods were validated and the specificity was assessed by analyzing synthetic mixtures containing the two drugs. The three methods were applied for the determination of the cited drug in tablets and the obtained results were statistically compared with those of a reported method. The comparison showed that there is no significant difference between the proposed methods and the reported method regarding both accuracy and precision.

Keywords: Atenolol; ratio subtraction; bivariate; area under curve.