Abstract
STABILITY-INDICATING SPECTROPHOTOMETRIC METHODS FOR DETERMINATION OF TORSEMIDE IN PURE FORM AND PHARMACEUTICAL PREPARATION

Khalid Abdel-Salam M. Attia, Nasr M. El-Abasawy and Rady F. Abdul-Kareem*

ABSTRACT

Six simple, rapid, sensitive, accurate and precise stability-indicating spectrophotometric methods were developed for the determination of Torsemide in bulk powder and in pharmaceutical preparation. Method (A), Ratio difference method; is based on measuring the difference in the amplitude of intact Torsemide in presence of its degradation product at two different wavelengths ,this is done at 236 nm and 270 nm in the range of 2.5 – 25 μg ml-1 with LOD = 0.325 μg ml-1 and LOQ = 0.986μg ml-1., Method (B), Mean centering method; the method was applied for analysis of Torsemide in presence of its degradation product this is done at 250 nm in the range of 5-25 μg ml-1 with LOD = 0.127 μg ml-1 and LOQ = 0.387 μg ml-1., Method (C) Bivariate method, is used for the determination of intact Torsemide in presence of its degradation product at 275&285 nm in the range of 5-25μg ml-1 with LOD= 0.532 μg ml-1 and LOQ= 1.613 μg ml-1 at (275 nm) and with LOD= 0.349 μg ml-1 and LOQ= 1.057 μg ml-1 at (285 nm) ., Method (D), First derivative of zero order spectra method (1D); is used for the determination of intact Torsemide in presence of its degradation product at 258.8 nm in the range of 5-25 μg ml-1 with LOD= 0.345μg ml-1 and LOQ= 1.046μg ml-1., Method (E), Dual wavelength method (DW) ; is based on measuring the difference in absorbance of intact Torsemide in presence of its degradation product at two different wavelengths in which degradate absorbance difference at these wavelengths is zero ,this is done at 265.4 nm and 296.6 nm in the range of 5-22.5 μg ml-1 with LOD= 0.115 μg ml-1 and LOQ= 0.349 μg ml-1., Method (F), Area under acurve (AUC) of zero order spectra method ; in which selection of the wavelength ranges which give good selectivity and percentage recovery 247–267 nm (λ1-λ2) and 270–296 nm (λ3-λ4) of Torsemide and its degradate , using Cramer's Rule in the concentration range of 5-22.5 μg ml-1 for each drug and its degradate with LOD=0.108 and LOQ=0.329 . The obtained results were statistically compared with those of the reported method by applying t-test and F-test at 95% confidence level and no significant difference was observed regarding accuracy and precision.

Keywords: Torsemide, Ratio difference, Mean centering, Bivariate, First derivative of zero order spectra, Dual wavelength, Area under acurve, pure form, harmaceutical preparation.


[Full Text Article]   [Download Certificate]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Dr. Dhrubo Jyoti Sen

Download Article : Click here

News & Updation

  • EJBPS: MAY ISSUE PUBLISHED

    MAY 2024 Issue has been successfully launched on 1 MAY 2024.

  • EJBPS New Impact Factor

    EJBPS Impact Factor has been Increased to 7.482 for Year 2024.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT